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Image Search Results
Journal: Journal of Cellular and Molecular Medicine
Article Title: MFTZ-1 reduces constitutive and inducible HIF-1α accumulation and VEGF secretion independent of its topoisomerase II inhibition
doi: 10.1111/j.1582-4934.2009.00822.x
Figure Lengend Snippet: Effects of MFTZ-1 on Akt and MAPK pathways in MDA-MB-231 cells. Cells were exposed to MFTZ-1 for 6 hrs at hypoxia (A), pre-starved MDA-MB-231 cells were exposed to MFTZ-1 for 4 hrs plus pre-stimulation with EGF (50 ng/ml) for 4 hrs at normoxia (B) before Western blotting analyses were done for the protein levels of HIF-1α, p-Akt, p-Erk1/2, p-p70s6k and p-4EBP1, Akt and Erk1/2. Data shown were representative of three independent experiments. (C) This scheme shows the relationship of Akt, Erk, p70s6k and 4EBP.
Article Snippet: The primary antibodies for p-Erk (#9101), Erk (#9102), p-Akt (#9272), Akt (#9271),
Techniques: Western Blot
Journal: Physiological Reports
Article Title: Impact of resistance exercise on ribosome biogenesis is acutely regulated by post‐exercise recovery strategies
doi: 10.14814/phy2.12670
Figure Lengend Snippet: Effect of resistance exercise on the p38‐ MNK 1‐ eIF 4E axis. Phosphorylation levels of p38 (A), MNK 1 (B), and eIF 4E (C), and total levels of eIF 4E (D), total cyclin D1 protein (E), and mRNA levels (F). Representative Western blot figures (closest molecular weight marker is shown in the left‐hand side) (G). Western blot data were normalized to GAPDH , with the exception of eIF 4E Ser209, which was normalized to its respective total protein. Cyclin D1 mRNA expression was normalized to the geometric mean of three reference genes. Values are mean ± SEM . *different from PRE within the same trial ( P < 0.05), # different between trials within the same time point ( P < 0.05). Main effects and interactions are presented in the text.
Article Snippet: The following antibodies were used in this study: total UBF (#13125), p‐UBF Ser484 (#21638) and Ser388 (#21637), cyclin D1 (#450), total eIF4E (#9976) (Santa Cruz Biotechnology, Santa Cruz, CA), total TIF‐IA (#42539), p‐TIF Ser649 (#138651), GAPDH (#36840)(Abcam, Cambridge, U.K.), total Akt (#2920), p‐Akt Thr308 (#4056), total PRAS40 (#2691), p‐PRAS40 Thr246 (#2691), p‐p38 MAPK Thr180/Tyr182 (#4511),
Techniques: Western Blot, Molecular Weight, Marker, Expressing